Device

Part:BBa_K1913015:Design

Designed by: Linea Katharina Muhsal   Group: iGEM16_Wageningen_UR   (2016-09-28)


Cry3Aa with araC/pBAD


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1219
    Illegal SpeI site found at 3266
    Illegal PstI site found at 3280
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1219
    Illegal NheI site found at 1205
    Illegal SpeI site found at 3266
    Illegal PstI site found at 3280
    Illegal NotI site found at 1225
    Illegal NotI site found at 3273
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1219
    Illegal BamHI site found at 1144
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1219
    Illegal suffix found in sequence at 3266
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1219
    Illegal XbaI site found at 1234
    Illegal SpeI site found at 3266
    Illegal PstI site found at 3280
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

-


Source

Bacillus thuringiensis var. tenebrionis

References